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KMID : 0384119930130040607
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 4 p.607 ~ p.616
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Comparison of Polymerase Chain Reaction and Viral Culture for the Detection of Cytomegalovirus
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Abstract
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Polymerase chain reaction (PCR) amplification is now widely used for the direct detection of etiologic agents from clinical specimens, and this method is clinically useful for those organisms requiring quite long time for conventional cultures,
such as
mycobacteria and viruses. This study was performed to confirm reliability of PCR method in the detection of CMV infection. Using urine samples from 72 patients, CMV DNA sequences were amplified by capillary PCR(rapid cycle amplification) using
two
sets
of CMV primer pairs and the results were compared to those of the culture method It took two to four hours to get results of CMV PCR amplification, whereas viral culture required two to twenty-eight days. There were 46 positive and 23 negative
samples
with concordant results from both PCR amplification and viral culture methods and the concordance of the two methods was 95.8%. Three of PCR-positive cases were initially equivocal-negative in virus culture and proved to be positive by repeated
cultures. PCR method turned out to be more sensitive than virai culture method. According to these results, PCR amplification is considered to be a very reliable and sensitive method for a rapid diagnosis of CMV infection.
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KEYWORD
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